Murine and rat cavernosal responses to endothelin-1 and urotensin-II Vasoactive Peptide Symposium

Abstract 

Endothelin-1 (ET-1) and urotensin-II (U-II) are the most potent constrictors of human vessels. Although the cavernosal tissue is highly responsive to ET-1, no information exists on the effects of U-II on cavernosal function. The aim of this study was to characterize ET-1 and U-II responses in corpora cavernosa from rats and mice. Male Wistar rats and C57/BL6 mice were used at 13 weeks. Cumulative concentration-response curves to ET-1, U-II, and IRL-1620, an ET_B agonist, were performed. ET-1 increased force generation in cavernosal strips from mice and rats, but no response to U-II was observed in the presence or absence of N^ω-nitro-L-arginine methyl ester (L-NAME), or in strips prestimulated with 20 mM KCl. IRL-1620 did not induce cavernosal contraction even in presence of L-NAME, but induced a cavernosal relaxation that was greater in rats than mice. No relaxation responses to U-II were observed in cavernosal strips precontracted with phenylephrine. mRNA expression of ET-1, ET_A, ET_B, and U-II receptors, but not U-II was observed in cavernosal strips. ET-1, via ET_A receptors activation, causes contractile responses in cavernosal strips from rats and mice, whereas ET_B receptor activation produces relaxation. Although the cavernosal tissue expresses U-II receptors, U-II does not induce contractile responses in corpora cavernosa from mice or rats.

Keywords: ET_A receptor, DOCA-salt hypertension, erectile dysfunction, corpus cavernosum