Journal of the American Society of Hypertension
Volume 2, Issue 6 , Pages 410-417 , November 2008

Increased vascular O-GlcNAcylation augments reactivity to constrictor stimuli — Vasoactive Peptide Symposium

  • Victor V. Lima

      Affiliations

    • Department of Physiology, Medical College of Georgia, Augusta, Georgia, USA
    • Corresponding Author InformationCorresponding author: Victor V. Lima, Medical College of Georgia, Department of Physiology, 1120 15th Street, CA-3141, Augusta, Georgia 30912. Tel: 706-721-0784; fax: 706-721-7299
    • ,
  • Fernanda R.C. Giachini, MSc

      Affiliations

    • Department of Physiology, Medical College of Georgia, Augusta, Georgia, USA
    • Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
    • ,
  • Fernando S. Carneiro, MSc

      Affiliations

    • Department of Physiology, Medical College of Georgia, Augusta, Georgia, USA
    • Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
    • ,
  • Zidonia N. Carneiro

      Affiliations

    • Department of Physiology, Medical College of Georgia, Augusta, Georgia, USA
    • ,
  • Zuleica B. Fortes, PhD

      Affiliations

    • Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
    • ,
  • Maria Helena C. Carvalho, PhD

      Affiliations

    • Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
    • ,
  • R. Clinton Webb, PhD

      Affiliations

    • Department of Physiology, Medical College of Georgia, Augusta, Georgia, USA
    • ,
  • Rita C. Tostes, PhD

      Affiliations

    • Department of Physiology, Medical College of Georgia, Augusta, Georgia, USA
    • Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil

Received 5 March 2008 ,Accepted 6 June 2008.

References 

  1. Hart GW, Housley MP, Slawson C. Cycling of O-linked β-N-acetyl-glucosamine on nucleocytoplasmic proteins. Nature. 2007;446:1017–1022
  2. Fülöp N, Marchase RB, Chatham JC. Role of protein O-linked N-acetyl-glucosamine in mediating cell function and survival in the cardiovascular system. Cardiovasc Res. 2007;73:288–297
  3. Wells L, Vosseller K, Hart GW. Glycosylation of nucleocytoplasmic proteins: signal transduction and O-GlcNAc. Science. 2001;291:2376–2378
  4. Marshall S, Bacote V, Traxinger RR. Discovery of a metabolic pathway mediating glucose-induced desensitization of the glucose transport system (Role of hexosamine biosynthesis in the induction of insulin resistance). J Biol Chem. 1991;266:4706–4712
  5. Goldberg HJ, Whiteside CI, Hart GW, Fantus IG. Posttranslational, reversible O-glycosylation is stimulated by high glucose and mediates plasminogen activator inhibitor-1 gene expression and Sp1 transcriptional activity in glomerular mesangial cells. Endocrinology. 2006;147:222–231
  6. Du XL, Edelstein D, Dimmeler S, Ju Q, Sui C, Brownlee M. Hyperglycemia inhibits endothelial nitric oxide synthase activity by posttranslational modification at the Akt site. J Clin Invest. 2001;108:1341–1348
  7. Musicki B, Kramer MF, Becker RE, Burnett AL. Inactivation of phosphorylated endothelial nitric oxide synthase (Ser1177) by O-GlcNAc in diabetes-associated erectile dysfunction. Proc Natl Acad Sci USA. 2005;102:11870–11875
  8. Kamemura K, Hayes BK, Comer FI, Hart GW. Dynamic interplay between O-glycosylation and O-phosphorylation of nucleocytoplasmic proteins: alternative glycosylation/phosphorylation of Thr58, a known mutational hot spot of c-Myc in lymphomas, is regulated by mitogens. J Biol Chem. 2002;277:19229–19235
  9. Chen L, Liu Y, Cui B, Mi Q, Huang Y, Fan L, et al. 17β-estradiol partially attenuates the inhibition of nitric oxide synthase-3 by advanced glycation end-products in human platelets. Clin Exp Pharmacol Physiol. 2007;34:972–978
  10. Rengifo J, Gibson CJ, Winkler E, Collin T, Ehrlich BE. Regulation of the inositol 1,4,5-trisphosphate receptor type I by O-GlcNAc glycosylation. J Neurosci. 2007;27:13813–13821
  11. Whitworth CE, Macauley MS, Stubbs KA, Dennis RJ, Taylor EJ, Vocadlo DJ, et al. Analysis of PugNAg and NAG-thiazoline as transition state analogues for human O-GlcNAcase: mechanistic and structural insights into inhibitor selectivity and transition state poise. J Am Chem Soc. 2007;129:635–644
  12. Patti ME, Virkamaki A, Landaker EJ, Kahn CR, Yki-Jarvinen H. Activation of the hexosamine pathway by glucosamine in vivo induces insulin resistance of early postreceptor insulin signaling events in skeletal muscle. Diabetes. 1999;48:1562–1571
  13. Vosseller K, Wells L, Lane MD, Hart GW. Elevated nucleocytoplasmic glycosylation by O-GlcNAc results in insulin resistance associated with defects in Akt activation in 3T3-L1 adipocytes. Proc Natl Acad Sci U S A. 2002;99:5313–5318
  14. Federici M, Menghini R, Mauriello A, Hribal ML, Ferrelli F, Lauro D, et al. Insulin-dependent activation of endothelial nitric oxide synthase is impaired by O-linked glycosylation modification of signaling proteins in human coronary endothelial cells. Circulation. 2002;106:466–472
  15. Hebert LF, Daniels MC, Zhou J, Crook ED, Turner RL, Simmons ST, et al. Overexpression of glutamine:fructose-6-phosphate aminotransferase in transgenic mice leads to insulin resistance. J Clin Invest. 1996;98:930–936
  16. Virkamaki A, Yki-Jarvinen H. Allosteric regulation of glycogen synthase and hexokinase by glucosamine-6-phosphate during glucosamine-induced insulin resistance in skeletal muscle and heart. Diabetes. 1999;48:1101–1107
  17. Parker G, Taylor R, Jones D, McClain D. Hyperglycemia and inhibition of glycogen synthase in streptozotocin-treated mice: role of O-linked N-acetyl-glucosamine. J Biol Chem. 2004;279:20636–20642
  18. Lehman DM, Fu DJ, Freeman AB, Hunt KJ, Leach RJ, Johnson-Pais T, et al. A single nucleotide polymorphism in MGEA5 encoding O-GlcNAc selective N-acetyl-beta-d glucosaminidase is associated with type 2 diabetes in Mexican Americans. Diabetes. 2005;54:1214–1221
  19. Ren J, Davidoff AJ. Diabetes rapidly induces contractile dysfunctions in isolated ventricular myocytes. Am J Physiol Heart Circ Physiol. 1997;272:H148–H158
  20. Dutta K, Carmody MW, Cala SE, Davidoff AJ. Depressed PKA activity contributes to impaired SERCA function and is linked to the pathogenesis of glucose-induced cardiomyopathy. J Mol Cell Cardiol. 2002;34:985–996
  21. Ren J, Gintant GA, Miller RE, Davidoff AJ. High extracellular glucose impairs cardiac E-C coupling in a glycosylation-dependent manner. Am J Physiol Heart Circ Physiol. 1997;273:H2876–H2883
  22. Hu Y, Belke D, Suarez J, Swanson E, Clark R, Hoshijima M, et al. Adenovirus-mediated overexpression of O-GlcNAcase improves contractile function in the diabetic heart. Circ Res. 2005;96:1006–1013
  23. Clark RJ, McDonough PM, Swanson E, Trost SU, Suzuki M, Fukuda M, et al. Diabetes and the accompanying hyperglycemia impairs cardiomyocyte calcium cycling through increased nuclear O-GlcNAcylation. J Biol Chem. 2003;278:44230–44237
  24. Zachara NE, O'Donnell N, Cheung WD, Mercer JJ, Marth JD, Hart GW. Dynamic O-GlcNAc modification of nucleocytoplasmic proteins in response to stress (A survival response of mammalian cells). J Biol Chem. 2004;279:30133–30142
  25. Savoia C, Schiffrin EL. Vascular inflammation in hypertension and diabetes: molecular mechanisms and therapeutic interventions. Clin Sci. 2007;112:375–384
  26. Kneass ZT, Marchase RB. Neutrophils exhibit rapid agonist-induced increases in protein-associated O-GlcNAc. J Biol Chem. 2004;279:45759–45765
  27. Daniels MC, McClain DA, Crook ED. Transcriptional regulation of transforming growth factor beta1 by glucose: investigation into the role of the hexosamine biosynthesis pathway. Am J Med Sci. 2000;319:138–142
  28. Mount PF, Kemp BE, Power DA. Regulation of endothelial and myocardial NO synthesis by multi-site eNOS phosphorylation. J Mol Cell Cardiol. 2007;42:271–279
  29. Li SY, Sigmon VK, Babcock SA, Ren J. Advanced glycation end-product induces ROS accumulation, apoptosis, MAP kinase activation and nuclear O-GlcNAcylation in human cardiac myocytes. Life Sci. 2007;80:1051–1056
  30. Guinez C, Mir AM, Leroy Y, Cacan R, Michalski JC, Lefebvre T. Hsp70-GlcNAc-binding activity is released by stress, proteasome inhibition, and protein misfolding. Biochem Biophys Res Commun. 2007;361:414–420
  31. Minamino T, Komuro I. Vascular cell senescence (Contribution to atherosclerosis). Circ Res. 2007;100:15–26
  32. Lakatta EG. Arterial and cardiac aging: major shareholders in cardiovascular disease enterprises (Part III: cellular and molecular clues to heart and arterial aging). Circulation. 2003;107:490–497

 This study was supported by the National Institutes of Health (HL74167), Bethesda, Maryland and CAPES, Brazil.

 VII International Symposium Vasoactive Peptide Symposium, February 14–16, 2008, Belo Horizonte, Brazil.

 Conflict of interest: none.

PII: S1933-1711(08)00129-0

doi: 10.1016/j.jash.2008.06.001

Journal of the American Society of Hypertension
Volume 2, Issue 6 , Pages 410-417 , November 2008

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